Deciphering molecular and functional codes of human memory lymphocytes

Abstract Long-term immunity against infections and effective response to vaccination are dependent on unique feature of lymphocytes referred as “memory generation”. Majority our understanding is established murine data human naive memory differentiation limited. In ongoing study we explored epigenetics (DNA methylation accessible chromatin), transcriptomics (basal, activation proliferation induced gene expression), intracellular cytokine assay (high-parameter flow cytometry) B T subsets from the healthy donors. We epigenetic changes associated with differentiation. However, relationship between transcriptomic outcomes didn’t explain entirely properties being or naïve lymphocyte. To understand mechanistic differences, cells CD4 were activated in vitro using either antigen receptor (BCR TCR crosslinking antibodies) PMA+Ionomycin (pan-activation) assayed for early late expression profiles. Surprisingly, majority patterns similar while their profiles significantly different. These findings indicate significant role translational machinery influencing lymphocyte functions. Our results direction segregating transcription translation mediated dependencies adaptive memory. will provide foundation future studies related dysregulation cell function aging infections. This project was supported by intramural funding NIA-NIH.